It is the first time that packages of livestreaming matches have been offered by the Premier League, under a strategy introduced by the chief executive, Richard Scudamore.
Amazon gets 20 games per season but its entry is significant because it marks the first time a streaming service has broadcast United Kingdom top-flight football live.
Amazon has bought one of the two remaining Premier League broadcast packages beginning in the 2019/20 season.
In addition Prime members can watch weekly highlights of all Premier Leagues games throughout the season.
Amazon is making its first foray into the broadcast of live Premier League football in the UK.
The US company is the first to break up the previous dominance of Sky and BT Sport.
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Sky Sports and BT Sport purchased 160 matches per season for approximately £4.5 billion earlier in the year, meaning that two packages remained unsold until this week, with one being midweek and the other being Bank Holiday fixtures. It also adds to Amazon's growing list of sports rights in Britain, which include US Open tennis.
The amount Amazon paid to secure exclusive Premier League footage has yet to be disclosed.
A fraught divide between the clubs in recent years had centred on leading clubs demanding a greater share of the global revenue stream.
Amazon and Twitter, along with Facebook (FB) and the YouTube unit of Alphabet (GOOGL), have been aggressive in trying to strike deals with sport leagues. The huge influx of cash - several times what had previously been paid for equivalent rights - helped Premier League clubs lure top players from all over the world.
What does this mean for the future of Premier League broadcasting?
"This new agreement will continue that trend with a subtle change that further incentivises on-pitch achievement and maintains the Premier League's position as the most equitable in Europe in terms of sharing central revenues".
Sky will now air 128 games a season, up from 126 matches currently and it now has the first pick game every weekend plus Saturday evening fixtures for the first time, all screened on Sky's dedicated Premier League channel.