The majority of that net worth comes from the 78.9 million shares of Amazon stock he owns.
Bezos is the largest shareholder of Amazon, the world's biggest online retailer. That eclipses the record previously held by Microsoft founder Bill Gates, who is now in second place with a net worth of $93.3 billion.
He and Bill Gates traded the title of world's richest person several times a year ago.
Amazon's share price, and therefore Bezos' net worth, has jumped significantly since September.More news: Gmail Brings Google Wallet To The UK
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Bezos also controls closely held space exploration business Blue Origin and the Washington Post.
Amazon shares rose 6.6 percent in 2017 and the company secured 89 percent of the online US spending market this holiday season among dominant stores.
Gates' previous record of richest person ever was set in April 1999, when he passed the $100 billion mark during the internet stock bubble. Adjusted for inflation, that would be $148 billion today.
Bezos came under fire past year when The New York Times questioned his level of philanthropic giving.