Dr Raja said he also found traces of VX nerve agent on Jong Nam's face, eyes, and on his bag and the blazer he wore.
Government chemist Raja Subramaniam told the court he found VX acid, a byproduct of the banned chemical weapon, on Siti Aisyah's sleeveless T-shirt.
Dr Nik Mohd Adzrul Ariff Raja Azlan, 34, who was the doctor on duty at the Menara Medical Clinic at the Kuala Lumpur International Airport 2 (klia2) on 13 February has testified in court on Monday on what happened after Jong-nam realised he had been poisoned.
It said a chemical analysis detected VX as well as VX precursors and by-products on Kim's body and clothing and luggage.
The women have pleaded not guilty, saying they thought they were involved in some sort of prank for a reality TV show.
Dr. Mohamad Shah, one of two pathologists who examined Kim's body and prepared the post-mortem report, said the cause of death was determined to be "acute VX nerve agent poisoning".
New Yorker journalist Evan Osnos (playing himself in the film) lays out the two basic theories, offense or defense, but I frankly can't think of any reason other than a glorious suicide wish that Kim Jong-Un or any other North Korean leader would want to initiate a first-use of nuclear weapons either against South Korea or against the United States.
Ri did not feature on the Interpol red notice issued in March which detailed four male North Korean suspects linked to the murder of Kim - all are believed to have left Malaysia after the attack was carried out and have been named as Ri Jae Nam, O Jong Gil, Hong Song Hak and Ri Ji Hyon.
The half-brother of North Korea's leader suffered extensive organ damage after being attacked with a nerve agent, a pathologist said on Tuesday, as more chilling details emerged at the trial of his alleged killers. He said Malaysia's airports do not have the special equipment needed to detect VX.More news: United States vs Panama CONCACAF WC Qualifiers
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He said VX is oily and hard to detect because it is colorless and odorless, and can be easily transported in a water bottle.
Government doctor Ranjini Sivaganabalan, who specializes in poison, later testified that VX may not be fatal in very low dosages.
VX is banned by an global treaty as a weapon of mass destruction but is believed to be part of North Korea's chemical weapons arsenal.
"The last person who wants conflict on the (Korean peninsula) is Kim Jong-un", Yong Suk Lee, deputy assistant director of the CIA's Korea Mission Center said.
"There's a clarity of goal in what Kim Jong-un has done", said Yong Suk Lee, deputy assistant director of the CIA's Korea Mission Centre, at an agency conference in George Washington University on Wednesday.
The North Korean dictator is infamous for executing his own officers, threatening the U.S. with nuclear war and increasing hostile long-range missile tests - the latest of which prompted harsh new global sanctions by the UN. The order came after Raja told the court the VX might still be active and it would be safer to view samples in the lab.
Prosecutors also sought Wednesday to show VX was the sole cause of death.
Traces of degradation elements were also discovered on the fingernail samples obtained from Doan.