Fittingly, he will face Justin Gatlin and the United States in the final chapter of his unparalleled career.
Even as he posed for all the selfies afterwards, demonstrating a grace and sportsmanship that proved him just as big a victor in rare defeat, it still felt wrong, a desperately downbeat way to depart for a sportsman who has brought so much joy to all.
"It is just one of those things, thank you to London for all your love and appreciation", said Bolt, who was treated by the capacity crowd and the organizers as a victor, receiving undimished cheers and applause from the adoring spectators and doing the lap of honor as if he had won.
For the best part of the past decade, though, Bolt has been 100 percent reliable in getting relay gold for the island nation.
One final time, Usain Bolt peered down the last 50 meters of his lane and saw sprinter upon sprinter running footsteps ahead of him.
American sprinter, Justin Gatlin, has accused the media of sensationalizing his rivalry with 8-time Olympic champion, Usain Bolt.
This year, however, he only got bronze in the 100 behind Gatlin. Gatlin's dramatic win will dominate these games long after their conclusion. The Americans beat Britain and Japan, who also qualified.
"My team did a great job", said Gatlin, the oldest of the squad at 35. I think he can break the national record next year in Australia (In CWG).
Christian Coleman took silver in the 100 ahead of Bolt and it gave the USA team an added boost.More news: Guardiola: Intelligent Bernardo Silva ready to make immediate Manchester City impact
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Gatlin, who actually trailed Bolt at the halfway point, heard boos cascade loudly across the stadium when his winning time, 9.92 seconds, popped up on the scoreboard.
Always a fan favorite, Bolt maintained his Hollywood smile as he jogged around the track following the race, Jamaican flag in tow. Trailing when he got the baton on the anchor leg, Bolt powered away, quickly swept past his rivals and had time to look to his right before crossing in 37.95 seconds. For he is just one of a suspicious number of Bolt's Jamaican teammates who, like Gatlin, have returned to competition after testing positive for PEDs.
Sir Mohamed Muktar Jama Farah is yet to official receive his gong at Buckingham Palace, that will come later in the year when he finally has time to breathe and reflect on his career. "It doesn't change anything for me", Bolt said.
Those quicksilver feet which always dealt in gold have another chance to obey his command as he seeks to bring down the curtain on his matchless sprint career and land a 20th global championship title in the 4 x 100 metres relay.
On the men's side, Usain Bolt is anticipated to line-up for Jamaica in Heat 2, alongside 110m hurdles champion Omar McLeod against teams such as Canada, China and France.
The four - the same line-up who won Team GB's first 4x100m relay bronze in 32 years in Rio last summer - crossed the line 0.09 seconds behind the U.S., with Germany qualifying as third fastest. They also advanced to the final. Bolt now holds the world record of 9.58 seconds, set during the 2009 World Athletics Championships finals in Berlin, Germany.
Felix has won 14 medals at the world championships and can move up to 16 if she runs the 4x400 relay and the United States wins medals in both events. He was banned for two years in 2001 when he was still at college. His 9.85-second run was the third fastest in Olympic history.
Whatever happens when Mo Farah steps on to the track for the final time at a major championships on Saturday night, he will leave it knowing no British athlete has been so staggeringly successful - or had their achievements so forcibly questioned.