The Broncos are already strapped for defensive line depth after losing reserve Billy Winn to a torn ACL on Thursday night in their preseason opener against the Chicago Bears.
Veteran defensive end Derek Wolfe went down with an ankle injury in the morning practice and had to be carted off the field.
Wolfe was helped onto a cart, driven off the practice field, and helped into the trainer's room while putting no weight on his right leg.
Wolfe, a leader and a versatile inside force, appeared to tangle legs with starting right tackle Menelik Watson on a running play moments after team drills started.
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In 11 practices open to the public, a record average of 4,910 fans came to watch the Broncos.
Nose tackle Kyle Peko is now tending to a broken foot. Strong safety T.J. Ward has been out with a hamstring injury. Winn's injury created a path for Shebly Harris to make the team. Outside linebackers Shane Ray (wrist) and Shaquil Barrett (hip) aren't expected back until the start of the regular season or later.
Wolfe had 51 tackles and 5.5 sacks past year and is a huge part of the Broncos run defense and pass rush.
Jared Crick is a returning starter at the other defensive end position.
Free-agent addition Zach Kerr and second-year defensive lineman Adam Gotsis will figure to get more work with the first-team for however long Wolfe is on the shelf.