The song had been played at some of the funerals of the people who lost their lives during the attack.
Held in the Emirates Old Trafford cricket ground, 50,000 thousand people stood silent as Marcus Mumford kicked off proceedings with a poignant moment of remembrance before the United Kingdom and the world were treated to three hours of performances include from Coldplay, Katy Perry, Justin Bieber, Pharrell, Niall Horan and more.
Grande's manager, Scooter Braun, said Sunday that the concert will go on as planned.
NEW YORK (AP) - Ariana Grande will return to the stage Sunday in Manchester, England, for a concert to raise money for victims of a suicide bombing that struck at the end of her last show almost two weeks ago.
Grande wore a One Love Manchester sweatshirt - and numerous fans wore shirts bearing similar messages, or face paint saying "I heart MCR", or #WeStandTogether stickers.
"I want to thank you from the bottom of my heart for being here today", the teary-eyed 23-year-old songstress added to the tens of thousands of fans from across the globe descended on Manchester's Old Trafford Cricket Ground for the benefit concert, noting that the event had to be revamped because of Saturday's terror assault on London.
She even collaborated with others onstage: She sang Fergie's verse on the Black Eyed Peas hit, "Where Is the Love?" along with the group; she performed a duet with Cyrus; and she sang her debut song, "The Way", with rapper Mac Miller.
This was Ariana's first stage appearance since the attacks on May 22. "The only thing we'll feel here tonight is love, and positivity".More news: EPA's Pruitt says leaving Paris Accord is about jobs, not politics
More news: Ottawa won't comment further about Canadian killed in London attack
More news: Police arrest 17th person in Manchester bombing
She was at the gig attacked by a suicide bomber on 22 May, killing 22 people and injuring many more.
The singer vowed to stay strong, sharing that she would return to the "incredibly fearless city" for a special performance.
What do you really think a jihadi will do, Katy, if you walked up to him and touched him and looked in his eyes and told him you love him?
The big event began with a moment's silence for those who lost their lives in the bomb blast, before folk star Marcus Mumford got proceedings underway by telling the audience to "not be afraid" ahead of his solo performance of Mumford & Sons' Timshel. Seven people were killed and 48 injured in the attack.
"Last night, this nation was challenged and all of you were challenged, and you had a decision to make if you were going to come out here tonight", he said.
When Grande ended the concert with a rendition of "Somewhere Over the Rainbow".